GMO molecular characterization details

GMO detailed information

GMO:
MON88302 x Rf3
Alias:
UID:
MON-883Ø2-9 x ACS-BNØØ3-6
Tradename:

InVigor, TruFlex, Roundup Ready
Companies/Institutions:
Bayer CropScience, Bayer Crop Science (formerly Monsanto Company)
Species:
Brassica napus
(canola, oilseed rape, rapeseed)
Traits:
EU authorisation:

no Reference materials could be found

Detection Methods

34 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-CP1/pFMV2	construct-specific	GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11).	ring trial validation	national standard	1
QL-CON-PGS-bar-A2/PGS-bar-B2	construct-specific	Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea)	Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13).	ring trial validation	national standard	1
QL-CON-00-008	construct-specific	2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-AgroBS2 F/AgroBS2 R	element-specific	V-Flanking Ti-ocs-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	1
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-BstarF-n2 /BstarR-n2	element-specific	CS-barstar	Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-epsps 1-5'/epsps 3-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-epsps2 1-5'/epsps2 1-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-P-SSuAra-F/P-SSuAra-R	element-specific	P-rbcS-ARATH	Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013)	in-house validation	none	1
QL-ELE-P-TA29-F/P-TA29-R	element-specific	P-ta29-TOBAC	Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-010	element-specific	E-FMV, P-34S FMV	Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-015	element-specific	E-FMV, P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5).	ring trial validation	ISO standard	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-019	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-024	element-specific	T-rbcS_E9-PEA	Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QL-TAX-BN-003	taxon-specific	Brassica napus	Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-BN-004	taxon-specific	Brassica napus	Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007).	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-EVE-BN-003	event-specific	Rf3	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-EVE-BN-010	event-specific	MON88302	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014)	ring trial validation	EU reference method	1
QT-TAX-BN-001	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014) Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-BN-002	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4	ring trial validation	EU reference method	1
QT-TAX-BN-003	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020)	ring trial validation	EU reference method	1
QT-TAX-BN-012	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-FatA primer1/FatA primer2	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	in-house validation	none	1

	Application reference number	Species	GMO	Intended use and EU authorisation status	Applicant	Expiry date
1	EFSA-GMO-NL-2013-119	Brassica napus (canola, oilseed rape, rapeseed)	MON88302 x Rf3	food feed cultivation other	Monsanto Europe	20.12.2027
2	GMFF-2022-11270 AP180	Brassica napus (canola, oilseed rape, rapeseed)	MON88302 x Rf3	food feed cultivation other	Bayer CropScience
3	EFSA-GMO-NL-2020-167	Brassica napus (canola, oilseed rape, rapeseed)	MON88302 x Rf3	food feed cultivation other	BASF Agricultural Solutions Seed US

Citation	Publication year	Publishing authority	Publication type	Content types
Commission Implementing Decision (EU) 2024/389 of 26 January 2024 renewing the authorisation for the placing on the market of products containing, consisting of or produced from genetically modified oilseed rape Ms8, Rf3 and Ms8 × Rf3 pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council	2024	EU	EU Commission Decision	decision document
Assessment of genetically modified oilseed rape MS8, RF3 and MS8 x RF3 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-024)	2023	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for authorisation to place on the market MON 94100 × MON 88302 × RF3 oilseed rape and its subcombinations independently of their origin in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed. GMFF-2022-11270 AP180, Part VII - Summary	2023	EFSA	application summary	application summary
Request for Renewal of the Authorization of the genetically modified herbicide tolerant canola Ms8, Rf3 and Ms8 x Rf3 for food and feed uses, and import and processing, in accordance with articles 11 and 23 of Regulation (EC) No 1829/2003, EFSA-GMO-RX024, Part III - Summary	2021	EFSA	application summary	application summary
Request for Authorisation of genetically modified herbicide tolerant MS11 x RF3 x MON88302 canola for food and feed uses, and import and processing, in accordance with articles 5 and 17 of Regulation (EC) No 1829/2003, EFSA-GMO-NL-2020-167, Part VII Summary	2020	EFSA	application summary	application summary
Commission Implementing Decision (EU) 2019/1301 of 26 July 2019 amending Implementing Decision 2013/327/EU as regards the renewal of the authorisation to place on the market feed containing or consisting of genetically modified oilseed rapes Ms8, Rf3 and Ms8 × Rf3 pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2019) 5499) (Text with EEA relevance.) C/2019/5499	2019	EU	EU Commission Decision	decision document
Request for Authorization of genetically modified herbicide tolerant RF3 Canola Quality Brassica juncea for food and feed uses, and import and processing, in accordance with Regulation (EC) No 1829/2003, EFSA-GMO-NL-2019-158, Part VII, Summary	2019	EFSA	application summary	application summary
Overall opinion of the European Food Safety Authority on genetically modified oilseed rape MS8, RF3 and MS8×RF3 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-004)	2017	EFSA	EFSA overall opinion	risk assessment document
Overall opinion of the European Food Safety Authority on application (EFSA-GMO-NL-2013-119) by Monsanto Company and Bayer CropScience AG for placing on the market genetically modified oilseed rape MON 88302 × MS8 × RF3 and subcombinations, independently of their origin for food and feed uses, import and processing in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 European Food Safety Authority	2017	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion on application EFSA-GMO-NL-2013-119 for authorisation of genetically modified glufosinateammonium- and glyphosate-tolerant oilseed rape MON 88302 3 MS8 3 RF3 and subcombinations independently of their origin, for food and feed uses, import and processing submitted in accordance with Regulation (EC) No 1829/2003 by Monsanto Company and Bayer CropScience	2017	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Assessment of genetically modified oilseed rape MS8, RF3 and MS8xRF3 for renewal of authorisation under regulation (EC) No 1829/2003 (application EFSA-GMO-RX-004)	2017	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
COMMISSION IMPLEMENTING DECISION (EU) 2017/2453 of 21 December 2017 authorising the placing on the market of products containing, consisting of, or produced from genetically modified oilseed rapes MON 88302 × Ms8 × Rf3 (MON-883Ø2-9 × ACSBNØØ5-8 × ACS-BNØØ3-6), MON 88302 × Ms8 (MON-883Ø2-9 × ACSBNØØ5-8) and MON 88302 × Rf3 (MON-883Ø2-9 × ACS-BNØØ3-6) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council on genetically modified food and feed (notified under document C(2017) 9045)	2017	EU	EU Commission Decision	decision document
Application for authorization to place on the market MON 88302 × MS8 × RF3 oilseed rape in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed Part VII - Summary	2017	EFSA	application summary	application summary
COMMISSION IMPLEMENTING DECISION (EU) 2015/687 of 24 April 2015 authorising the placing on the market of products containing, consisting of, or produced from genetically modified oilseed rape MON 88302 (MON-883Ø2-9) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2015) 2759)	2015	EU	EU Commission Decision	decision document
PCR detection of the pSSUAra-bar construct for screening of genetically modified oilseed rape lines - construct-specific method. BVL Official Method Collection G 30.40-13 Online Publication (2015)	2015		other document	detection
Application for authorization to place on the market MON 88302 oilseed rape in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed Part VII - Summary	2014	EFSA	application summary	application summary
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-BE-2011-101) for the placing on the market of herbicide tolerant genetically modified oilseed rape MON 88302 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto	2014	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion on application (EFSA-GMO-BE-2011-101) for the placing on the market of herbicide-tolerant genetically modified oilseed rape MON 88302 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto	2014	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Commission implementing decision of 25 June 2013 authorising the placing on the market of food containing or consisting of genetically modified oilseed rape Ms8, Rf3 and Ms8 × Rf3, or food and feed produced from those genetically modified organisms pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2013) 3873) (2013/327/EU)	2013	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-BE-2010-81) for the placing on the market of the genetically modified herbicide-tolerant oilseed rape Ms8, Rf3 and Ms8 x Rf3 for food containing or consisting of, and food produced from or containing ingredients produced from genetically modified oilseed rape Ms8, Rf3 and Ms8 x Rf3 (with the exception of processed oil) under Regulation (EC) No 1829/2003 from Bayer European Food Safety Authority	2012	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion on application (EFSA-GMO-BE-2010-81) for the placing on the market of genetically modified herbicide-tolerant oilseed rape Ms8, Rf3 and Ms8 × Rf3 for food containing or consisting of, and food produced from or containing ingredients produced from, oilseed rape Ms8, Rf3 and Ms8 × Rf3 (with the exception of processed oil) under Regulation (EC) No 1829/2003 from Bayer EFSA Panel on Genetically Modified Organisms (GMO)	2012	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Request for Authorization of Glufosinate Ammonium-Tolerant Genetically Modified Oilseed Rape Ms8, Rf3 and Ms8xRf3 in accordance with article 5 of Regulation (EC) No 1829/2003. Part II - Summary	2012	EFSA	application summary	application summary
Scientific Opinion on the revised molecular characterisation for Rf3 oilseed rape received from the Competent Authority of Belgium under Article 20(3) of Directive 2001/18/EC1 (Application C/BE/96/01)	2010	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on the application (reference EFSA-GMO-RX-Ms8-Rf3) for renewal of the authorisation of existing products produced from herbicide tolerant genetically modified oilseed rape Ms8 x Rf3 for food and feed uses from Bayer CropScience AG European Food Safety Authority	2009	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion on an application (EFSA-GMO-RX-MS8-RF3) for renewal of the authorisation for continued marketing of existing (1) food and food ingredients produced from genetically modified glufosinate-tolerant oilseed rape Ms8, Rf3 and Ms8 x Rf3, and (2) feed materials produced from genetically modified glufosinate-tolerant oilseed rape Ms8, Rf3 and Ms8 x Rf3, under Regulation (EC) No 1829/2003 from Bayer CropScience EFSA Panel on Genetically Modified Organisms (GMO Panel)	2009	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Summary of the request for renewal, according to Articles 11 and 23, of the authorisations notified under Articles 8(1)(A) and 20(1)(B) of Regulation (EC) No. 1829/2003 - Glufosinate ammonium-tolerant genetically modified oilseed rape Ms8/Rf3 for derived food and feed products Part II - Summary	2008	EFSA	application summary	application summary
Savini C., Bogni A., Mazzara M., Van Den Eede G.; Event-specific Method for the Quantification of Oilseed Rape Line Rf3 Using Real-time PCR - Validation Report and Protocol - Seeds Sampling and DNA Extraction Online Publication (2007)	2007		EU reference method protocol	detection
Commission Decision of 26 March 2007 concerning the placing on the market, in accordance with Directive 2001/18/EC of the European Parliament and of the Council, of oilseed rape products (Brassica napus L., lines Ms8, Rf3 and Ms8xRf3) genetically modified for tolerance to the herbicide glufosinate-ammonium, 2007/232/EC	2007	EU	EU Commission Decision	decision document
Opinion of the Scientific Panel on Genetically Modified Organisms on a request from the Commission related to the application (Reference C/BE/96/01) for the placing on the market of glufosinate-tolerant hybrid oilseed rape Ms8 x Rf3, derived from genetically modified parental lines (Ms8, Rf3), for import and processing for feed and industrial uses, under Part C of Directive 2001/18/EC from Bayer CropScience (Question No EFSA-Q-2005-003)	2005	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document

European GMO Initiative for a Unified Database System

GMO detailed information

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