GMO molecular characterization details

GMO detailed information

GMO:
GMB471
Alias:

EE-GM4, SCN resistant soybean
UID:
BCS–GM471–2
Tradename:
Companies/Institutions:
Bayer CropScience, BASF Agricultural Solutions Seed US LCC
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert_GMB471 (putative)
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the T-DNA of plasmid pSZ8832.
Map:

Genetic Elements:

Reference: patent documents

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888		length: 270 bp; counter clockwise
2	CS-cry14Ab1-BACTU	Bacillus thuringiensis	-	Chimeric gene coding for the delta-endotoxin Cry14Ab1	Nematode resistance	length: 3558 bp; counter clockwise
3	P-ubi10-ARATH	Arabidopsis thaliana	-	Promoter of the Ubiquitin-10 gene. Norris et al. (1993). Plant Mol Biol, 21(5):895-906		length 1307 bp; counter clockwise
4	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888		length: 195 bp; counter clockwise
5	CS-HPPD-PSEFL	Pseudomonas fluorescens	-	sequence encoding a 4-hydroxyphenylpyruvate dioxygenase variant. Patent US6245968B1		length: 1077 bp; counter clockwise
6	TP-rbcS-SYNTH	Synthetic	-	Optimised chloroplast transit peptide derivative of the RuBisCo small unit genes from Zea mays and Helianthus annuus		length: 372 bp; TPotpY-lPf: coding sequence of an optimized transit peptide derivative (position 55 changed into Tyr), containing sequence of the RuBisCO small subunit genes of Zea mays and Helianthus annuus (US Patent 5510471); counter clockwise.
7	L-TEV	Tobacco etch virus	-			length: 125 bp; counter clockwise
8	P-e35S-CaMV	Cauliflower mosaic virus	-	Cauliflower mosaic virus (CaMV) 35S promoter with duplicated enhancer region; inserted element length can vary between e.g. 307bp/322bp/296bp/551bp/615bp; Kay R, et al. (1987) Science 236, 1299-1302		length: 750 pb; counter clockwise

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

patent documents No

Transformation Vectors

Name:	vector_pZ88832
Comment:
Map:

Genetic Elements:

Reference: patent documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-intervening-SYNTH	Synthetic	130	1-130
2	T-35S-CaMV	Cauliflower mosaic virus	270	131-400	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888		counter clockwise
3	V-intervening-SYNTH	Synthetic	11	401-411
4	CS-cry14Ab1-BACTU	Bacillus thuringiensis	3558	412-3969	Chimeric gene coding for the delta-endotoxin Cry14Ab1	Nematode resistance	counter clockwise
5	P-ubi10-ARATH	Arabidopsis thaliana	1307	3970-5276	Promoter of the Ubiquitin-10 gene. Norris et al. (1993). Plant Mol Biol, 21(5):895-906		counter clockwise
6	V-intervening-SYNTH	Synthetic	105	5277-5381
7	T-35S-CaMV	Cauliflower mosaic virus	195	5382-5576	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888		counter clockwise
8	V-intervening-SYNTH	Synthetic	12	5577-5588
9	CS-HPPD-PSEFL	Pseudomonas fluorescens	1077	5589-6665	sequence encoding a 4-hydroxyphenylpyruvate dioxygenase variant. Patent US6245968B1		counter clockwise
10	TP-rbcS-SYNTH	Synthetic	372	6666-7037	Optimised chloroplast transit peptide derivative of the RuBisCo small unit genes from Zea mays and Helianthus annuus		TPotpY-lPf: coding sequence of an optimized transit peptide derivative (position 55 changed into Tyr), containing sequence of the RuBisCO small subunit genes of Zea mays and Helianthus annuus (US Patent 5510471). counter clockwise
11	V-intervening-SYNTH	Synthetic	21	7038-7058
12	L-TEV	Tobacco etch virus	127	7059-7185			counter clockwise
13	V-intervening-SYNTH	Synthetic	6	7186-7191
14	P-e35S-CaMV	Cauliflower mosaic virus	750	7192-7941	Cauliflower mosaic virus (CaMV) 35S promoter with duplicated enhancer region; inserted element length can vary between e.g. 307bp/322bp/296bp/551bp/615bp; Kay R, et al. (1987) Science 236, 1299-1302		counter clockwise
15	V-intervening-SYNTH	Synthetic	127	7942-8068

Related DNA Sequences

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	patent documents	No

Detection Methods

21 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-023	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Related DNA Sequences

Detection Methods

Reference Materials

Authorisation

Literature reference