GMO molecular characterization details

GMO detailed information

GMO:
EH92-527-1
Alias:

Amflora
UID:
BPS-25271-9
Tradename:
Companies/Institutions:
BASF, Amylogene
Species:
Solanum tuberosum
(potato)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_EH92-527-1
Comment:	Insert order indicates order of genetic elements as described for cassettes present in T-DNA. Order and copy number of genetic elements in T-DNA is not clear. During the integration process, DNA sequences towards the left border region of the T-DNA were deleted and the entire T-DNA insert, including the flanking region, was duplicated in reverse orientation prior to integration. The insert comprises the 5’ untranslated region of the nopaline synthase gene (P-nos-RHIRD); nptII coding sequence; 3’ untranslated part of nopaline synthase gene (T-nos-RHIRD); potato gbss promoter fragment; a fragment of gbss coding region in antisense orientation. The insert, including the potato flanking region, was duplicated in a tail-to-tail arrangement with two right border regions as junctions to the potato chromosomal DNA.
Map:

Genetic Elements:

Reference: EFSA Journal

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens		-
2	P-nos-RHIRD	Agrobacterium tumefaciens		-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
3	CS-nptII-ECOLX	Escherichia coli		-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
4	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
5	P-gbss-SOLTU	Solanum tuberosum		-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30
6	CS-gbss_RNAi_as-SOLTU	Solanum tuberosum		-	Fragment of the granule-bound starch synthase gene (gbss) in antisense orientation	Amylose/amylopectin ratio
7	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
8	V-LB-RHIRD	Agrobacterium tumefaciens		-
	V-Flanking Ti-nos-RHIRD	Agrobacterium tumefaciens		-	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the nopaline type; sequence located in the promoter of nopaline synthase.

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

EFSA Journal No

Detection Methods

16 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-014	construct-specific	1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2	Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4)	ring trial validation	ISO standard	2
QL-ELE-AgroBS1 F/AgroBS1 R	element-specific	V-Flanking Ti-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	3
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	2
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	2
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	3
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	3
QT-EVE-ST-001	event-specific	EH92-527-1	Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014)	ring trial validation	EU reference method	3
QT-TAX-ST-010	taxon-specific	Solanum tuberosum	Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference