GMO molecular characterization details

GMO detailed information

GMO:
CTC91087
Alias:
UID:
CTC-91Ø87-6
Tradename:
Companies/Institutions:
Centro de Tecnologia Canavieira (CTC)
Species:
Saccharum officinarum
(sugar cane, sugarcane)
Traits:
EU authorisation:

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No results found.
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General Information

Inserts / Modification

Name:	insert_CTC91087
Comment:	The expression of Cry1Ac in CTC91087 is regulated by the maize ubi-1 promoter and T-nos from Agrobacterium tumefaciens. The PAT (bar) expression in CTC91087 is also regulated by ubi-1 and T-nos.
Map:

Genetic Elements:

Reference: scientific journal

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	O-flanking DNA-HOST			-
2	V-intervening-SYNTH	Synthetic		-
3	P-ubi1-MAIZE	Zea mays		-	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
4	CS-cry1Ac-BACTU	Bacillus thuringiensis ssp. Kurstaki		-	Codon optimized gene or artificial gene incoding for (at least the insect-active portion of) the cry1Ac protein similar to the Bacillus thuringiensis var. kurstaki HD73. Adang et al. (1985) Gene 36:289-300; Fischhoff and Perlak (1996) U.S. patent 5500365	Lepidoptera resistance	codon optimized for sugarcane; encodes a 615 aa toxin with an estimated molecular weight of 68KDa and insecticidal tryptic core of 52 KDa.
5	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
6	V-intervening-SYNTH	Synthetic		-
7	P-ubi1-MAIZE	Zea mays		-	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
8	CS-bar-STRHY	Streptomyces hygroscopicus		-	Phosphinothricin acetyltransferase gene derived from the common soil bacterium Streptomyces hygroscopicus a.k.a. bar gene shares 85 per cent homology at the amino acid level with the pat gene. Thompson et al. (1987) EMBO J. 6:2519-2523	Glufosinate tolerance
9	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
10	V-intervening-SYNTH	Synthetic		-
11	O-flanking DNA-HOST			-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

scientific journal No

Detection Methods

14 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2	element-specific	CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-Ubi-F/P-Ubi-R	element-specific	P-ubi1-MAIZE	Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-016	element-specific	CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).	ring trial validation	ISO standard	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1

Authorisation

Literature reference