GMO molecular characterization details

GMO detailed information

GMO:
LLRICE62
Alias:

LL62
UID:
ACS-OSØØ2-5
Tradename:

LibertyLink
Companies/Institutions:
Bayer CropScience, AgrEvo
Species:
Oryza sativa
(rice)
Traits:
EU authorisation:

General Information

Inserts / Modification

Order	Name	Donor	Size (bp)	Position	Definition	Trait
1	O-flanking DNA-HOST		501	1-501
2	vector fragment		122	502-623
3	P-Cauliflower mosaic virus		529	624-1152
4	vector fragment		17	1153-1169
5	CS-bar-STRHY	Streptomyces hygroscopicus	552	1170-1721	Phosphinothricin acetyltransferase gene derived from the common soil bacterium Streptomyces hygroscopicus a.k.a. bar gene shares 85 per cent homology at the amino acid level with the pat gene. Thompson et al. (1987) EMBO J. 6:2519-2523	Glufosinate tolerance
6	vector fragment		18	1722-1739
7	T-Cauliflower mosaic virus		198	1740-1937
8	vector fragment		57	1938-1994
9	O-flanking DNA-HOST		500	1995-2494

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
KF036176	NCBI - GenBank	Yes	2494	KF036176\| Oryza sativa Indica Group transgenic cultivar Bengal phosphinothricin-acetyl-transferase (bar) gene, complete cds

Detection Methods

23 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-015	construct-specific	Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC)	in-house validation	none	2
QL-ELE-AINT 2-5'/AINT 2-3'	element-specific	I-actin	Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009).	in-house validation	none	3
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	3
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	3
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QL-TAX-OS-002	taxon-specific	Oryza sativa	Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006).	in-house validation	unknown	1
QL-TAX-OS-003	taxon-specific	Oryza sativa	Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005)	ring trial validation	national standard	1
QL-TAX-OS-006	taxon-specific	Oryza sativa	Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).	ring trial validation	unknown	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	3
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-EVE-OS-002	event-specific	LLRICE62	Quantitative real-time PCR (TaqMan) method for detection of rice event LLRICE62 (EU-RL GMFF GMOMETHODS database as of January 01, 2014)	ring trial validation	EU reference method	3
QT-TAX-OS-002	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016)	ring trial validation	EU reference method	1
QT-TAX-OS-003	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009)	ring trial validation	unknown	1
QT-TAX-OS-017	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	1
QT-TAX-SPS primer f/SPS primer r	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1)	ring trial validation	ISO standard	1

Name:	insert_LLRICE62
Comment:
Map:

Catalogue number	Source	Value	Type	Certified	Available
AOCS 0306-D2	AOCS	<0.01 % (m/m)	genomic DNA	yes	no
AOCS 0306-D3	AOCS	<0.01 % (m/m)	genomic DNA	yes	no
AOCS 0306-D5	AOCS	0 % (m/m)	dried powder	yes	yes
AOCS 0306-I4	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-I6	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-I9	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 1022-A	AOCS	0,0008 % (m/m)	dried powder	yes	yes
AOCS 1022-B	AOCS	>96.2 % (m/m)	dried powder	yes	yes
5211508501	Eurofins GeneScan	100 cp/µl	plasmid DNA	no	no
5211514102	Eurofins GeneScan	20 cp/µl	genomic DNA	no	no
5211514120	Eurofins GeneScan	20 cp/µl	genomic DNA	no	no

Citation	Publication year	Publishing authority	Publication type	Content types
1829/2003 LLRICE62 summary Part II FORMAT OF THE SUMMARY OF APPLICATIONS FOR GENETICALLY MODIFIED PLANTS AND/OR DERIVED FOOD AND FEED	2008	EFSA	application summary	application summary
Opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No. 1829/2003 on application EFSA-GMO-UK-2004-04 Application for the placing on the market of genetically modified LLRICE62 for food and feed uses from Bayer CropScience. (Question No. EFSA-Q-2004-145) 30 November 2007	2007	EFSA	EFSA overall opinion	risk assessment document
Opinion of the Scientific Panel on Genetically Modified Organisms on an application (reference EFSA-GMO-UK-2004-04) for the placing on the market of glufosinate tolerant genetically modified rice LLRICE62 for food and feed uses, import and processing, under Regulation (EC) No 1829/2003 from Bayer CropScience GmbH1 (No EFSA-Q-2004-145) Opinion adopted on 30 October 2007	2007	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference