GMO molecular characterization details

GMO detailed information

GMO:
Da tomato
Alias:

87-22A/08, 87-22/08
UID:
SYN-ØØØDA-9
Tradename:
Companies/Institutions:
Syngenta, Zeneca Seeds
Species:
Solanum lycopersicum
(tomato)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

Inserts / Modification

Name:	insert_Da tomato
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order and copy number of genetic elements in the insert is not clear.
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens		-			derived from Ti plasmid pTiT37
2	V-intervening-SYNTH	Synthetic		-			length: 562 bp, comprises a 406 bp-long fragment of the origin of replication (ori) from bacteriophage M13 and a 156 bp long fragment of CS-beta_gal-ECOLX of Escherichia coli strain ECO which is a derivative of E. coli K12
3	TP-nls-SYNTH	Synthetic		-	synthetic nuclear localisation sequence (NLS).		length: 529 bp
4	CS-pg_RNAi_s-SOLLC	Solanum lycopersicum		-	Fragment of the polygalacturonase (pg) gene from Lycopersicon esculentum var. Ailsa Craig in sense orientation. Grierson et al. (1986). Nucleic Acids Res, 14, 8595-8605; Smith et al. (1990). Plant Mol Biol, 14, 369-379	Altered ripening or flowering	length: 731 bp, first 731 bp of a cDNA clone that was derived from the native CS-pg-SOLLC of Solanum lycopersicum (Lycopersicon esculentum Mill. variety Ailsa Craig)
5	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		length: 247 bp
6	V-intervening-SYNTH	Synthetic		-			length: 671 bp; comprises a 231 bp-long fragment of CS-beta_gal-ECOLX of Escherichia coli strain ECO which is a derivative of E. coli K12 and a 440 bp long internal fragment of Gene III from bacteriophage M13 which is a component of the viral coat
7	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		length: 258 bp
8	V-intervening-SYNTH	Synthetic		-			length: 209 bp; internal fragment of ornithine cyclodeaminase gene (CS-ocd-RHIRD) and fragment of unspecified vector backbone
9	CS-nptII-ECOLX	Escherichia coli		-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance	length: 800 bp
10	P-nos-RHIRD	Agrobacterium tumefaciens		-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807		length: 227 bp
11	V-RB-RHIRD	Agrobacterium tumefaciens		-			derived from Ti plasmid pTiT37

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

application documents No

Detection Methods

16 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-014	construct-specific	1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2	Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-SL-001	taxon-specific	Solanum lycopersicum	Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005).	ring trial validation	ISO standard	1
QL-TAX-SL-005	taxon-specific	Solanum lycopersicum	Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008).	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-TAX-SL-002	taxon-specific	Solanum lycopersicum	Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1)	ring trial validation	ISO standard	1

Authorisation

Literature reference