1 |
V-RB-RHIRD |
Agrobacterium tumefaciens |
25 |
1-25 |
|
|
truncated |
2 |
vector fragment |
|
152 |
26-177 |
|
|
Ti Plasmid Region |
3 |
V-intervening-SYNTH |
Synthetic |
258 |
178-435 |
|
|
|
4 |
V-loxP-BPP1 |
Bacteriophage P1 |
34 |
436-469 |
|
|
|
5 |
V-intervening-SYNTH |
Synthetic |
253 |
9187-9439 |
|
|
|
6 |
vector fragment |
|
21 |
9440-9460 |
|
|
att4B: Bacteriophage lambda integrase recombination site |
7 |
V-intervening-SYNTH |
Synthetic |
158 |
9461-9618 |
|
|
|
8 |
T-In2_1-MAIZE |
Zea mays |
343 |
9619-9961 |
Terminator region from the Zea mays In2-1 gene. Hershey and Stoner (1991), Plant Mol Biol 17(4):679-90. doi: 10.1007/BF00037053. |
|
|
9 |
V-intervening-SYNTH |
Synthetic |
7 |
9962-9968 |
|
|
|
10 |
CS-amy-MAIZE |
Zea mays |
1334 |
9969-11302 |
Alpha-amylase gene (aa1). Young et al. (1994) Plant Physiol. 105(2):759-760 |
Male sterility |
truncated α-amylase gene including 3' untranslated region: 3’ UTR at bp 9,969-10,039 (length: 71 bp),
coding sequence at bp 10,040-11,302 (length: 1,263 bp); complementary |
11 |
TP-brittle1-MAIZE |
Zea mays |
227 |
11303-11529 |
|
|
amyloplast-targeting transit peptide of the Brittle-1 gene including 5’ untranslated region:
coding sequence at bp 11,303-11,527 (length: 225 bp)
5’ UTR at bp 11,528-11,529 (length: 2 bp); complementary |
12 |
P-pg47-MAIZE |
Zea mays |
2736 |
11530-14265 |
Promoter of the pollen polygalacturonase (Pg47) gene. Allen and Lonsdale (1993) Plant J, 3(2):261-271 |
|
complementary |
13 |
V-intervening-SYNTH |
Synthetic |
67 |
14266-14332 |
|
|
|
14 |
vector fragment |
|
24 |
14333-14356 |
|
|
attB1: Bacteriophage lambda integrase recombination site from the Invitrogen Gateway® cloning system |
15 |
V-intervening-SYNTH |
Synthetic |
35 |
14357-14391 |
|
|
|
16 |
E-35S-CaMV |
Cauliflower mosaic virus |
475 |
14392-14866 |
|
|
|
17 |
V-intervening-SYNTH |
Synthetic |
33 |
14867-14899 |
|
|
|
18 |
P-ltp2-HORVU |
Hordeum vulgare |
846 |
14900-15745 |
Promoter of a lipid transfer protein gene. Kalla (1994). The Plant Joumal, 6(6):849-860 |
|
|
19 |
V-intervening-SYNTH |
Synthetic |
44 |
15746-15789 |
|
|
|
20 |
CS-DsRed2-SYNTH |
Synthetic |
678 |
15790-16467 |
Variant with one alteration (Alt1) of Clontech's DsRed protein, isolated from a marine coral, Discosoma sp., with unchanged amino acid sequence |
DsRed2 activity |
|
21 |
V-intervening-SYNTH |
Synthetic |
41 |
16468-16508 |
|
|
|
22 |
T-pinII-SOLTU |
Solanum tuberosum |
311 |
16509-16819 |
3' untranslated region (3' UTR) of the proteinase inhibitor II gene; 0.31kb terminator sequence. |
|
|
23 |
V-intervening-SYNTH |
Synthetic |
121 |
16820-16940 |
|
|
|
24 |
vector fragment |
|
24 |
16941-16964 |
|
|
attB2: Bacteriophage lambda integrase recombination site from the Invitrogen Gateway® cloning System |
25 |
V-intervening-SYNTH |
Synthetic |
41 |
16965-17005 |
|
|
|
26 |
P-ms44-MAIZE |
Zea mays |
1221 |
17006-18226 |
Promoter region from the male-sterile mutant gene ms44. Fox et al. (2017). Plant Biotechnol J, 15, 942-952. doi: 10.1111/pbi.12689. |
|
|
27 |
V-intervening-SYNTH |
Synthetic |
3 |
18227-18229 |
|
|
|
28 |
CS-ms44 5' precursor 396h_RNAi-MAIZE |
Zea mays |
82 |
18230-18311 |
5' precursor sequence of the microRNA backbone 396h from Zea mays. Patent WO 2009079548. Patent WO2009079532. |
Fertility restoration |
|
29 |
CS-ms44 amiRNA sequence_RNAi-MAIZE |
Zea mays |
21 |
18312-18332 |
Artificial microRNA (amiRNA) sequence complementary to the ms44 gene from Zea mays. Patent WO 2009079548. Fox et al. (2017). Plant Biotechnol J, 15, 942-952. doi: 10.1111/pbi.12689 |
Fertility restoration |
|
30 |
CS-ms44 precursor 396h_RNAi-MAIZE |
Zea mays |
68 |
18333-18400 |
Precursor sequence of the microRNA backbone 396h. Patent WO 2009079548. Patent WO2009079532. |
Fertility restoration |
|
31 |
CS-ms44 star Sequence_RNAi-MAIZE |
Zea mays |
21 |
18401-18421 |
Artificial star sequence complementary to the ms44 amiRNA sequence except for one mismatched nucleotide. Patent WO 2009079548. Fox et al. (2017). Plant Biotechnol J, 15, 942-952. doi: 10.1111/pbi.12689 |
Fertility restoration |
|
32 |
CS-ms44 3' precursor 396h_RNAi-MAIZE |
Zea mays |
441 |
18422-18862 |
3' precursor sequence of the microRNA backbone 396h from Zea mays. Patent WO 2009079548. Patent WO2009079532. |
Fertility restoration |
|
33 |
V-intervening-SYNTH |
Synthetic |
18 |
18863-18880 |
|
|
|
34 |
T-ms44-MAIZE |
Zea mays |
775 |
18881-19655 |
Terminator region from the male-sterile mutant gene ms44. Fox et al. (2017). Plant Biotechnol J, 15, 942-952. doi: 10.1111/pbi.12689. |
|
|
35 |
V-intervening-SYNTH |
Synthetic |
98 |
19656-19753 |
|
|
|
36 |
vector fragment |
|
21 |
19754-19774 |
|
|
attB3: Bacteriophage lambda integrase recombination site |
37 |
V-intervening-SYNTH |
Synthetic |
225 |
19775-19999 |
|
|
|
38 |
vector fragment |
|
57 |
20000-20056 |
|
|
Ti Plasmid Region |
39 |
V-LB-RHIRD |
Agrobacterium tumefaciens |
25 |
20057-20081 |
|
|
Truncated |