Description:
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GE-PT4 rice lines were generated by CRISPR/Cas9 technology targeting the phosphate transporter gene pt4 in Oryza sativa Nipponbare.
The phosphate transporter PT4 is involved in arsenate (As(V)) uptake and transport in rice.
Aim of the mutation of the pt4 gene was to generate low As-accumulating rice.
For gene editing of pt4, a Cas9 vector was introduced into Oryza sativa Nipponbare via Agrobacterium tumefaciens-mediated transformation.
Two mutant lines were obtained.
In GE-PT4 plants As accumulation in roots decreased 17–30% compared with WT Nipponbare.
Source: Ye et al. (2017), OsPT4 Contributes to Arsenate Uptake and Transport in Rice, Frontiers in Plant Science 8, 2197.
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Links regarding to this GMO:
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Scientific publication - Ye et al. (2017) https://www.frontiersin.org/articles/10.3389/fpls.2017.02197/full
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Transformation / Modification technique:
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CRISPR/Cas
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