Description:
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GE-CD163 pigs were generated using CRISPR/Cas9 gene editing in pig zygotes.
Porcine reproductive and respiratory syndrome virus (PRRSV; new name: Betaarterivirus suid 1) is causing late-term abortions, stillbirths, and respiratory disease in pigs, leading to major economic losses to the worldwide pig industry. GE-CD163 pigs inoculated with PRRSV-1 (strain BOR-57) were resistant to viral infection.
In GE-CD163 pigs, exon 7 of the cd163 gene has been excised using two guide RNAs targeting the intron up- and downstream of exon 7. This results in the lack of the PRRS virus interaction site, the scavenger receptor cysteine-rich domain 5 (SRCR5) of the CD163 protein. cd163 expression and biological function of its protein remained unchanged.
Heterozygous pigs were bred to yield homozygous GE-CD163 animals.
Sources:
- Burkard et al. (2018), Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection. Journal of Virology 92:e00415-18.
- Burkard et al. (2017), Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function. PLOS Pathogens 13:e1006206.
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Links regarding to this GMO:
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Scientific publication - Burkard et al. (2018) https://journals.asm.org/doi/full/10.1128/JVI.00415-18,
Scientific publication - Burkard et al. (2017) https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006206
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Transformation / Modification technique:
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CRISPR/Cas
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