Description:
|
PT73 Escherichia coli (TM) consists of a genetically modified strain of E. coli K-12 named strain AG3139.
Strain AG3139 has been constructed from the E. coli K12 strain MG1655 in several steps. The strategy of construction was based, among others, on chromosomal insertion for the genes introduced. Except for one modification, the only genes transferred in the final strain AG3139 correspond to the over-expression of existing metabolic enzymes for the synthesis/production of amino acids.
The purpose of the genetic modifications is to obtain a high production rate of L-threonine.
Southern blots have shown that none of the antibiotic resistance genes used for intermediate constructions (tetracycline, kanamycin, streptomycin, chloramphenicol and bleomycin) are present in the genome of strain AG3139.
Further hybridizations have shown the stability of the copies integrated in the genome even after 15 to 20 generations.
Sources:
Application submitted under regulation (EC) No 1829/2003: EFSA-Q-2008-412b on Part II - Summary of EFSA-GMO-FR-2008-059
Afssa - Saisie n°2008-SA-0238
|
Links regarding to this GMO:
|
|
Transformation / Modification technique:
|
_unknown
|