QL-CON-CP1/pFMV2 |
construct-specific |
GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). |
ring trial validation |
national standard |
1 |
QL-CON-PGS-bar-A2/PGS-bar-B2 |
construct-specific |
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea) |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). |
ring trial validation |
national standard |
1 |
QL-CON-00-008 |
construct-specific |
2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-ELE-AgroBS2 F/AgroBS2 R |
element-specific |
V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation |
national standard |
1 |
QL-ELE-bar 2-5'/bar 2-3' |
element-specific |
CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) |
in-house validation |
none |
1 |
QL-ELE-B'nase-F-n4/BNaseR-n3 |
element-specific |
CS-barnase |
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013). |
in-house validation |
none |
1 |
QL-ELE-BstarF-n2 /BstarR-n2 |
element-specific |
CS-barstar |
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) |
in-house validation |
none |
1 |
QL-ELE-epsps 1-5'/epsps 3-3' |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). |
in-house validation |
none |
1 |
QL-ELE-epsps2 1-5'/epsps2 1-3' |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) |
in-house validation |
none |
1 |
QL-ELE-P-SSuAra-F/P-SSuAra-R |
element-specific |
P-rbcS-ARATH |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-P-TA29-F/P-TA29-R |
element-specific |
P-ta29-TOBAC |
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-T-g7-F/T-g7-R |
element-specific |
T-orf23-RHIRD, T-transcript 7 |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-00-006 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-007 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) |
ring trial validation |
national standard |
1 |
QL-ELE-00-009 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-010 |
element-specific |
E-FMV, P-34S FMV |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-011 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-013 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-014 |
element-specific |
CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-015 |
element-specific |
E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-018 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-019 |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-022 |
element-specific |
CS-bar-STRHY |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-024 |
element-specific |
T-rbcS_E9-PEA |
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). |
ring trial validation |
EU reference method |
1 |
QL-ELE-00-026 |
element-specific |
CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016). |
ring trial validation |
EU reference method |
1 |
QL-TAX-BN-003 |
taxon-specific |
Brassica napus |
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-TAX-BN-004 |
taxon-specific |
Brassica napus |
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). |
ring trial validation |
unknown |
1 |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' |
element-specific |
T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) |
ring trial validation |
none |
1 |
QT-EVE-BN-002 |
event-specific |
Ms8 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-BN-003 |
event-specific |
Rf3 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-BN-010 |
event-specific |
MON88302 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) |
ring trial validation |
EU reference method |
1 |
QT-TAX-BN-001 |
taxon-specific |
Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-BN-002 |
taxon-specific |
Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4 |
ring trial validation |
EU reference method |
1 |
QT-TAX-BN-003 |
taxon-specific |
Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) |
ring trial validation |
EU reference method |
1 |
QT-TAX-BN-012 |
taxon-specific |
Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-FatA primer1/FatA primer2 |
taxon-specific |
Brassica napus |
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
in-house validation |
none |
1 |