GMO molecular characterization details

GMO detailed information

GMO:
MON88913
Alias:
UID:
MON-88913-8
Tradename:

Genuity Roundup Ready Flex
Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company)
Species:
Gossypium hirsutum
(cotton)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_MON88913
Comment:	BCH Record ID 15168 http://bch.cbd.int/database/record.shtml?documentid=15168
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait
1	E-FMV	Figwort mosaic virus	-
2	P-EF1alpha-ARATH	Arabidopsis thaliana	-	Promoter of the elongation factor EF-1 alpha gene. Axelos et al. (1989). Mol Gen Genet, 219(2):106-112
3	L-EF1alpha-ARATH	Arabidopsis thaliana	-
4	I-1_EF1alpha-ARATH	Arabidopsis thaliana	-
5	TP-ctp2-ARATH	Arabidopsis thaliana	-
6	CS-CP4epsps-RHIRD	Agrobacterium tumefaciens ssp. CP4	-	5-enolpyruvylshikimate- 3-phosphate synthase gene. Steinruecken et al. (1980) Biochem Biophys Res Com. 94:1207-1212	Glyphosate tolerance
7	T-rbcS_E9-PEA	Pisum sativum	-	3' untranslated region (3' UTR) f the rinulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) gene. Coruzzi et al. (1984) EMBO J. 3:1671-1679
8	E-35S-CaMV	Cauliflower mosaic virus	-
9	P-act8-ARATH	Arabidopsis thaliana	-	Promoter of the actin 8 gene. An et al. (1996). The Plant Journal 10(1), 107-121
10	L-act8-ARATH	Arabidopsis thaliana	-
11	I-1_act8-ARATH	Arabidopsis thaliana	-
12	TP-ctp2-ARATH	Arabidopsis thaliana	-
13	CS-CP4epsps-RHIRD	Agrobacterium tumefaciens ssp. CP4	-	5-enolpyruvylshikimate- 3-phosphate synthase gene. Steinruecken et al. (1980) Biochem Biophys Res Com. 94:1207-1212	Glyphosate tolerance
14	T-rbcS_E9-PEA	Pisum sativum	-	3' untranslated region (3' UTR) f the rinulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) gene. Coruzzi et al. (1984) EMBO J. 3:1671-1679
	V-Flanking Ti-ocs-RHIRD	Agrobacterium tumefaciens	-	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the octopine type.

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No

Detection Methods

20 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-CP1/pFMV2	construct-specific	GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11).	ring trial validation	national standard	1
QL-CON-00-008	construct-specific	2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-AgroBS2 F/AgroBS2 R	element-specific	V-Flanking Ti-ocs-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-AINT 2-5'/AINT 2-3'	element-specific	I-actin	Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009).	in-house validation	none	1
QL-ELE-epsps2 1-5'/epsps2 1-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-PFMV 1-5'/PFMV 1-3'	element-specific	P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009)	in-house validation	none	3
QL-ELE-P-FMV-F/P-FMV-R	element-specific	P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013)	in-house validation	none	3
QL-ELE-00-010	element-specific	E-FMV, P-34S FMV	Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-015	element-specific	E-FMV, P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5).	ring trial validation	ISO standard	2
QL-ELE-00-019	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-024	element-specific	T-rbcS_E9-PEA	Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	3
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-EVE-GH-007	event-specific	MON88913	Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014)	ring trial validation	EU reference method	3
QT-TAX-GH-015	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-016	taxon-specific	Gossypium hirsutum	Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-018	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-019	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-020	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-021	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1

Description:	Cotton MON88913 was produced by Agrobacterium-mediated transformation. It was developed to produce a glyphosate-tolerant cotton by the introduction of the epsps coding sequence of Agrobacterium sp. strain CP4 encoding a 5-enolpyruvylshikimate-3-phosphate synthase protein (CP4 EPSPS). The T-DNA introduced in MON88913 was derived from plasmid PV-GHGT35, containing two CP4epsps expression cassettes in tandem orientation. The molecular characterisation data establish that the GM cotton MON88913 contains a single copy of the introduced DNA on a single locus of integration. No other parts of the plasmid used for transformation are present in cotton MON88913.
Links regarding to this GMO:	ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=56
Transformation / Modification technique:	Agrobacterium-induced

Catalogue number	Source	Value	Type	Certified	Available
AOCS 0804-A	AOCS	<0.4 % (m/m)	dried powder	yes	no
AOCS 0804-A2	AOCS	<0.08 % (m/m)	dried powder	yes	yes
AOCS 0906-D	AOCS	>98.51 % (m/m)	dried powder	yes	no
AOCS 0906-D2	AOCS	≥98.5 % (m/m)	dried powder	yes	yes
5211511401	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no

Citation	Publication year	Publishing authority	Publication type	Content types
COMMISSION IMPLEMENTING DECISION (EU) 2015/688 of 24 April 2015 authorising the placing on the market of products containing, consisting of, or produced from genetically modified cotton MON 88913 (MON-88913-8) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2015) 2760)	2015	EU	EU Commission Decision	decision document
SCIENTIFIC OPINION Statement complementing the EFSA opinion on application EFSA-GMO-UK-2007-41 (cotton MON 88913 for food and feed uses, import and processing) taking into consideration updated bioinformatic analyses	2014	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for authorization of MON 88913 cotton in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed Part II Summary	2013	EFSA	application summary	application summary
TECHNICAL REPORT Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-UK-2007-41) for the placing on the market of herbicide-tolerant genetically modified cotton MON 88913 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto	2013	EFSA	EFSA overall opinion	risk assessment document
SCIENTIFIC OPINION Scientific Opinion on application EFSA-GMO-UK-2007-41 for the placing on the market of herbicide-tolerant genetically modified cotton MON 88913 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto	2013	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference