GMO molecular characterization details

GMO detailed information

GMO:
DAS81910
Alias:
UID:
DAS-8191Ø-7
Tradename:
Companies/Institutions:
DOW AgroSciences
Species:
Gossypium hirsutum
(cotton)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_DAS81910
Comment:
Map:

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	O-genomic DNA-HOST		1373	1-1373
2	V-LB-RHIRD	Agrobacterium tumefaciens	2	1374-1375
3	V-intervening-RHIRD	Agrobacterium tumefaciens	136	1376-1511
4	E-rb7_mar-TOBAC		1166	1512-2677
5	V-MCS-SYNTH	Synthetic	95	2678-2772
6	P-ubi10-ARATH	Arabidopsis thaliana	1322	2773-4094	Promoter of the Ubiquitin-10 gene. Norris et al. (1993). Plant Mol Biol, 21(5):895-906
7	V-MCS-SYNTH	Synthetic	8	4095-4102
8	CS-aad12-DELAC	Delftia acidovorans	882	4103-4984	Aryloxyalkanoate dioxygenase 12 gene (aad12). Wright et al. (2010) PNAS 107(47):20240-20245	2,4-D tolerance
9	V-MCS-SYNTH	Synthetic	102	4985-5086
10	T-orf23-RHIRD	Agrobacterium tumefaciens	457	5087-5543	3' untranslated region (3' UTR) of the ORF23 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350
11	V-MCS-SYNTH	Synthetic	114	5544-5657
12	P-CsVMV	Cassava vein mosaic virus	517	5658-6174	Promoter region of the Cassava vein mosaic virus. Verdaguer et al. (1996). Plant Mol Biol, 31:1129-1139.
13	V-MCS-SYNTH	Synthetic	7	6175-6181
14	CS-pat-STRVR	Streptomyces viridochromogenes	552	6182-6733	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37	Glufosinate tolerance
15	V-MCS-SYNTH	Synthetic	102	6734-6835
16	T-orf1-RHIRD	Agrobacterium tumefaciens	704	6836-7539	3' untranslated region (3' UTR) of the ORF1 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350
17	V-intervening-RHIRD	Agrobacterium tumefaciens	224	7540-7763
18	O-genomic DNA-HOST		1071	7764-8834

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

USDA-APHIS No

Detection Methods

12 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-Patf-n2/Patr-n2	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	3
QL-ELE-00-021	element-specific	CS-pat-STRVR	Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-025	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016.	ring trial validation	EU reference method	3
QT-ELE-00-002	element-specific	CS-pat-STRVR	Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020)	ring trial validation	unknown	2
QT-EVE-GH-011	event-specific	DAS81910	Quantitative PCR method for detection of cotton event DAS81910 (EURL GMFF GMOMETHODS database as of January 10, 2019)	ring trial validation	EU reference method	1
QT-TAX-GH-015	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-016	taxon-specific	Gossypium hirsutum	Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-018	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-019	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-020	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-021	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference