GMO molecular characterization details

GMO detailed information

GMO:
MON87701
Alias:
UID:
MON-877Ø1-2
Tradename:
Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company)
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_MON87701
Comment:	Sequence comparison to the parental soybean A5547 indicated that in soybean MON87701, a 32 bp DNA segment of endogenous DNA had been deleted and 14 bp have been introduced immediately 5' of the insertion site.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	P-rbcS-ARATH	Arabidopsis thaliana		-	Promoter of the ribulose- 1,5-bisphosphate carboxylase (Rubisco) small subunit gene. Almeida et al. (1989) Mol Gen Genet, 218(1):78-86
2	TP-rbcS-ARATH	Arabidopsis thaliana		-
3	CS-cry1Ac-BACTU	Bacillus thuringiensis ssp. Kurstaki		-	Codon optimized gene or artificial gene incoding for (at least the insect-active portion of) the cry1Ac protein similar to the Bacillus thuringiensis var. kurstaki HD73. Adang et al. (1985) Gene 36:289-300; Fischhoff and Perlak (1996) U.S. patent 5500365	Lepidoptera resistance
4	T-7Salpha-SOYBN	Glycine max		-	3' region encoding the 7S alpha seed storage protein (conglycinin), incl. 35 nucleotides of the carboxyl terminal beta-conglycinin coding region with termination codon and polyadenylation sequence. Schuler et al. (1982) Nuc Acid Res. 10(24):8245-8261
	V-LB-RHIRD	Agrobacterium tumefaciens		-
	V-RB-RHIRD	Agrobacterium tumefaciens		-
	V-Flanking Ti-ocs-RHIRD	Agrobacterium tumefaciens		-	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the octopine type.

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
HB386793	NCBI - GenBank	Yes	14416	Sequence 6 from Patent WO2009064652, sequence of event MON87701 (including amplicon for method QT-EVE-GM-010)
RIKILT_MON87701_event	Wageningen Food Safety Research (WFSR)	Yes	89	Amplicon sequence of MON87701 for event-specific method QT-EVE-GM-010 without probe
RIKILT_P-SSuAra_element_MON87701	Wageningen Food Safety Research (WFSR)	Yes	95	Amplicon sequence of MON87701 for element-specific method QL-ELE-P-SSuAra-F/P-SSuAra-R without probe
RIKILT_cry1Ac_2_element_MON87701	Wageningen Food Safety Research (WFSR)	Yes	74	Amplicon sequence of MON87701 for element-specific method QL-ELE-00-016 without probe
RIKILT_cry1Ac_element_MON87701	Wageningen Food Safety Research (WFSR)	Yes	157	Amplicon sequence of MON87701 for element-specific method QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 without probe

Detection Methods

17 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-AgroBS2 F/AgroBS2 R	element-specific	V-Flanking Ti-ocs-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2	element-specific	CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013).	in-house validation	none	3
QL-ELE-P-SSuAra-F/P-SSuAra-R	element-specific	P-rbcS-ARATH	Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-016	element-specific	CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).	ring trial validation	ISO standard	3
QL-ELE-00-020	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-EVE-GM-010	event-specific	MON87701	Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87701 (EU-RL GMFF GMOMETHODS database as of January 23, 2014)	ring trial validation	EU reference method	3
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference