Description:
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hLZ goats were developed to produce human lysozyme (hLZ) in their milk, a protein which finds application in the food industry and in therapeutic applications.
hLZ goats were generated through pronuclear microinjection into goat zygotes. Goats from the founding animal (1 female) were used for further breeding and maintained as hemizygous transgenic goats. Transgenic offsprings were identified by PCR and confirmed by Southern blot.
The DNA construct used to generate this event consists of the promoter sequence of the bovine alpha-S1-casein gene (P-csn1s1-BOVIN), the 540 bp cDNA encoding the human lysozyme gene (CS-lyz-HUMAN) inserted into the second exon of the bovine alpha-S1-casein gene, followed by the 3’ regulatory sequence of alpha-S1-casein (T-csn1s1-BOVIN).
Expression under the alpha-S1-casein promoter (P-csn1s1-BOVIN) drives expression to the mammary gland (Maga et al., 1994).
Sources:
Maga et al. (1994), Expression of human lysozyme mRNA in the mammary gland of transgenic mice. Transgenic Research 3:36-42.
Maga et al. (2006), Production and Processing of Milk from Transgenic Goats Expressing Human Lysozyme in the Mammary Gland. Journal of Dairy Science 89(2):518-524.
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Links regarding to this GMO:
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Scientific publication - Maga et al., (1994) https://link.springer.com/article/10.1007%2FBF01976025,
Scientific publication – Maga et al., (2006) https://www.sciencedirect.com/science/article/pii/S0022030206721142?via%3Dihub
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Transformation / Modification technique:
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Reproductive cloning
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