Description:
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The Solanum lycopersicum event 'J2 Tomato' was developed by targeted knockout of MADS-box gene J2 (Solyc12g038510) using CRISPR/Cas9. MADS-box gene J2 encodes the MADS-box protein J2 (SIMBP21).
MADS-box protein J2 controls meristem maturation and inflorescence architecture, the knockout of this gene results in an easier abscission (disconnecting the fruit from the plant). Abscission has been shown to impact post-harvest and even improved fruit yield due to reduced loss-of-water and wounds.
Two natural variations of this MADS-box gene J2 knockout have been observed. These are caused by insertion of a Rider retrotransposable element and a missense stop-codon mutation.
CRISPR/Cas9 is transferred into Solanum lycopersicum with two sgRNAs targetting the third and fourth exons of MADS-box gene J2. Absence of the abscission zone in Solanum lycopersicum was confirmed phenotypically.
Source:
Roldan et al., (2017), Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato. Scientific Reports 7:4402.
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Links regarding to this GMO:
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UniProt entry of MADS-box protein J2 http://www.uniprot.org/uniprot/K4DEK0,
Scientific publication - Roldan et al., (2017) https://www.nature.com/articles/s41598-017-04556-1
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Transformation / Modification technique:
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CRISPR/Cas
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