QT-TAX-GH-021 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-020 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-019 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-018 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-016 |
taxon-specific |
Gossypium hirsutum |
Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-015 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-EVE-GH-012 |
event-specific |
COT102 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020) |
ring trial validation |
EU reference method |
1 |
QT-EVE-GH-005 |
event-specific |
MON15985 |
Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
ring trial validation |
EU reference method |
1 |
QT-ELE-00-004 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) |
ring trial validation |
ISO standard |
1 |
QT-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) |
ring trial validation |
unknown |
1 |
QT-ELE-P35S 1-5'/P35S 1-3' |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) |
ring trial validation |
national standard |
1 |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' |
element-specific |
T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) |
ring trial validation |
none |
1 |
QL-ELE-00-018 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-017 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-016 |
element-specific |
CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-013 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-012 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-011 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-009 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-007 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) |
ring trial validation |
national standard |
1 |
QL-ELE-00-006 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-005 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-004 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-003 |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) |
ring trial validation |
national standard |
1 |
QL-ELE-00-002 |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-npt 1-5'/npt 1-3' |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). |
in-house validation |
none |
1 |
QL-ELE-cry2Ab2 - F/cry2Ab2 - R |
element-specific |
CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). |
in-house validation |
none |
1 |
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 |
element-specific |
CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). |
in-house validation |
none |
1 |
QL-ELE-AgroBS1 F/AgroBS1 R |
element-specific |
V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation |
national standard |
1 |
QL-CON-35S-F/nptII-R |
construct-specific |
23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010). |
in-house validation |
none |
1 |