Description:
|
F10 was developed through Agrobacterium-mediated transformation of the potato variety Ranger Russet with plasmid vector pSIM1278. The intended consequence of the genetic modification in F10 is to silence genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes.
F10 was confirmed to be free of Agrobacterium-derived vector backbone DNA. Southern hybridizations showed that F10 contain a single DNA insert comprising all sequences needed for expression of both the Asn1/Ppo5 gene silencing cassette and R1/PhL promoter silencing cassette. Molecular analysis and an enzymatic assay indicated that the DNA insert was stable through at least the second generation.
|
Links regarding to this GMO:
|
|
Transformation / Modification technique:
|
Agrobacterium-induced
|