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Detection Image Detection method details

Name:

 QL-ELE-At-nop-f2/At-nop-r2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the nopaline synthase gene (nos) from Agrobacterium tumefaciens (BVL G30.40-16)

Comment:

 Target is the nopaline synthase gene (nos) from Agrobacterium tumefaciens (indicating the presence of Ti-plasmid from phytopathogenic Agrobacterium strains)

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    At-nop-f2

  • Sequence:

    ccagccrtstactgattattgtmac

  • Size:

    25

  • Reverse Primer

  • Name:

    At-nop-r2

  • Sequence:

    tgcgagttcrccgttgaag

  • Size:

    19

  • Probe

  • Name:

    At-nop-s1

  • Sequence:

    ccgtgcggacgttcacgacag

  • Size:

    21

  • Amplicon:

  • Sequence:

    ccagccgtgtactgattattgtaacccgtgcggacgttcacgacagcttcgtcaacgaactcgccaacttcaacggcgaactcgca

  • Size:

    86

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