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Detection Image Detection method details

Name:

QL-ELE-00-003

Description:

Qualitative conventional PCR method for detection of Neomycin phosphotransferase II gene (L 00.00-31, 2001)

Comment:

target is the Neomycin phosphotransferase II (nptII) gene

Validation:

ring trial validation

Standardisation:

national standard

Related Methods:

Type:

element-specific

Target DNA element:

CS-nptII-ECOLX, V-nptII-ECOLX
  • Oligonucleotides:

  • Forward Primer

  • Name:

    TN5-1
  • Sequence:

    GGATCTCCTGTCATCT
  • Size:

    16
  • Reverse Primer

  • Name:

    TN5-2
  • Sequence:

    GATCATCCTGATCGAC
  • Size:

    16
  • Amplicon:

  • Sequence:

    ggatctcctgtcatctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgatcaggatgatc
  • Size:

    173

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-ELE-00-003Link to the document url. EU reference method protocol
Screening procedure for the detection of genetically modified DNA sequences in foods by identification of DNA sequences that frequently occur in genetically modified organisms. BVL Official Method Collection L 00.00-31 Online Publication (2001)Link to the document url. other document 11-05-2016