Detection Image Detection methods search result

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QT-TAX-OS-002 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) ring trial validation EU reference method
QL-TAX-DC-001 taxon-specific
Dianthus caryophyllus
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). in-house validation EU reference method
QL-TAX-SL-001 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). ring trial validation ISO standard
QL-TAX-GM-002 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). ring trial validation ISO standard
QT-TAX-OS-003 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) ring trial validation unknown
QT-TAX-SL-002 taxon-specific
Solanum lycopersicum
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) ring trial validation ISO standard
QT-TAX-ZM-001 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcoholdeydrogenase 1 (adh1) gene.

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QL-BAC-16S-FW/16S-Rev taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QL-BAC-27f/926R taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QT-TAX-GM-001 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QL-TAX-SL-005 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). ring trial validation unknown
QT-TAX-ST-010 taxon-specific
Solanum tuberosum
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) ring trial validation EU reference method
QT-TAX-BN-002 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4

ring trial validation EU reference method
QT-TAX-BN-001 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QL-TAX-PS-001 taxon-specific
Pisum sativum
Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017) ring trial validation EU reference method
QL-TAX-CP-001 taxon-specific
Carica papaya
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) ring trial validation EU reference method
QL-TAX-GM-001 taxon-specific
Glycine max
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-BN-003 taxon-specific
Brassica napus
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-ZM-002 taxon-specific
Zea mays
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) ring trial validation unknown
QT-TAX-CP-001 taxon-specific
Carica papaya
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) ring trial validation EU reference method
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